| 高春生,王小伟,高俊,等.miR-27b对骨肉瘤MG63细胞生长的影响及其机制探讨.骨科,2022,13(1): 57-61. |
| miR-27b对骨肉瘤MG63细胞生长的影响及其机制探讨 |
| Effect of miR-27b on Growth of Osteosarcoma MG63 Cells and Possible Mechanism |
| 投稿时间：2021-02-28 |
| DOI：10.3969/j.issn.1674-8573.2022.01.013 |
| 中文关键词: miR-27b 骨肉瘤 细胞增殖 侵袭 迁移 凋亡 |
| 英文关键词: miR-27b Osteosarcoma Cell proliferation Invasion Migration Apoptosis |
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| 中文摘要: |
| 目的 探讨miR-27b对骨肉瘤MG63细胞生长的影响及其机制。方法 将体外培养的MG63细胞分为模拟物对照组、miR-27b模拟物组、抑制剂对照组和miR-27b抑制剂组，采用RT-PCR检测各组细胞中miR-27b的表达，MTT法检测细胞增殖活性，Transwell小室法检测细胞侵袭和迁移，流式细胞仪检测细胞凋亡。双荧光素酶报告基因实验检测miR-27b和FOXO1的靶向关系，Western blot检测FOXO1蛋白表达。结果 过表达miR-27b后，骨肉瘤MG63细胞增殖活性升高，侵袭和迁移数增加，而细胞凋亡率降低（P＜0.05）。抑制miR-27b表达与过表达miR-27b的作用相反。miR-27b靶向调控FOXO1，过表达miR-27b后FOXO1蛋白的表达水平降低，而抑制miR-27b表达后FOXO1蛋白的表达水平升高（P＜0.05）。结论 miR-27b可能通过靶向调控FOXO1促进骨肉瘤MG63细胞的增殖、侵袭和迁移，并抑制细胞凋亡。 |
| 英文摘要: |
| Objective To investigate the effect of miR-27b on the growth of osteosarcoma MG63 cells and the possible mechanism. Methods MG63 cells cultured in vitro were divided into mimic control group, miR-27b mimic group, inhibitor control group and miR-27b inhibitor group. The expression of miR-27b was detected by RT-PCR, cell proliferation was detected by MTT, cell invasion and migration were detected by Transwell chamber, and cell apoptosis was detected by flow cytometry. Double luciferase reporter gene assay was used to detect the targeting relationship between miR-27b and FOXO1, and the expression of FOXO1 protein was detected by Western blotting. Results After overexpression of miR-27b, proliferation activity of MG63 cells was increased, number of invasive and migrating cells increased, and apoptosis rate was decreased (P＜0.05). Inhibiting miR-27b expression had the opposite effect to overexpression of miR-27b. The miR-27b targeted and regulated FOXO1. The expression of FOXO1 protein was decreased after overexpression of miR-27b, while the expression of FOXO1 protein was increased after inhibiting miR-27b expression (P＜0.05). Conclusion The miR-27b may promote the proliferation, invasion and migration of MG63 cells and inhibit cell apoptosis by targeting FOXO1. |
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