| 焦占营,李洋,方纪成,等.正常雌雄大鼠椎间盘微细结构差异的MRI研究.骨科,2021,12(4): 358-361. |
| 正常雌雄大鼠椎间盘微细结构差异的MRI研究 |
| Characterizations of the Intervertebral Disc Microstructure in Male and Female Matured Rats by T2 Mapping and T2* Mapping |
| 投稿时间：2020-08-23 |
| DOI：10.3969/j.issn.1674-8573.2021.04.012 |
| 中文关键词: 大鼠 性别 T2 mapping T2* mapping 椎间盘 |
| 英文关键词: Rat Gender T2 mapping T2* mapping Intervertebral disc |
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| 中文摘要: |
| 目的 利用T2 mapping和T2* mapping探究雌雄性正常大鼠椎间盘微细结构的差异。方法 选用12只正常成年雌性SD大鼠和10只正常成年雄性SD大鼠，运用T2 mapping和T2* mapping技术对正常大鼠的第6、7尾椎椎间盘C6/7和第7、8尾椎椎间盘C7/8进行扫描，并通过画取感兴趣区域的方法获得相应椎间盘的T2值和T2*值。同时对椎间盘进行T2加权矢状位扫描，采用Pfirrmann分级法进行分级评价。最后对椎间盘进行病理检测，获取雌雄性大鼠的椎间盘病理切片结果。结果 雌性大鼠的24个椎间盘中有14个（58%）属于Ⅰ级，10个（42%）属于Ⅱ级；雄性大鼠的20个椎间盘中有14个（70%）属于Ⅰ级，6个（30%）属于Ⅱ级。在Ⅰ级和Ⅱ级椎间盘中，雌性大鼠的T2值和T2*值都明显小于雄性大鼠，差异均有统计学意义（P均＜0.05）。雄性大鼠的椎间盘形态较雌性大鼠细长，而椎间盘微细结构中，雄性大鼠椎间盘中的纤维含量比雌性大鼠要少。结论 不同性别正常成年大鼠的椎间盘存在明显差异，而且正常成年的雌雄性大鼠的椎间盘均可能发生退变，故在进行椎间盘相关的动物实验前，应当选择同性别实验大鼠进行实验，并对实验大鼠提前进行MRI检测，选取具有相同退变程度的实验大鼠。 |
| 英文摘要: |
| Objective To investigate the differences in the microstructure of the intervertebral discs between different genders. Methods Twelve normal female rats and 10 normal male rats were used in this study. T2 mapping and T2* mapping techniques were used in the intervertebral discs between the sixth and seventh (C6/7), seventh and eighth coccygeal vertebrae (C7/8) of mature rats to obtain the T2 value and T2* value. In addition, T2-weighted sagittal imaging was also performed on the intervertebral disc to get the discs' T2 grades by Pfirrmann grading system. Pathological examinations were performed on pathological section of the intervertebral disc from female and male rats to obtain the pathological results. Results Fourteen (58%) of the 24 intervertebral discs in female rats were classified as grade Ⅰ, and 10 (42%) discs as grade Ⅱ; 14 (70%) of the 20 discs in male rats belonged to grade Ⅰ, and 6 (30%) discs to grade Ⅱ. In grade I and Ⅱ intervertebral discs, the T2 and T2* values of female rats were significantly lower than those of male rats (P＜0.05). The intervertebral discs of male rats were more elongated, while the those of female rats were thicker and shorter. Female rats had more fiber in the intervertebral disc than male rats. Conclusion There is also some degenerative change in the intervertebral discs of mature rats. These rats should be detected before the test to make the consistence. There are significant differences in the intervertebral discs of mature rats in different genders. In the future studies, the rats with the same sex should be chosen. |
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