| 朱海燕,邹浩,甘一波.血管内皮生长因子及骨形态发生蛋白2对成骨细胞生长的促进作用.骨科,2014,5(2): 73-75. |
| 血管内皮生长因子及骨形态发生蛋白2对成骨细胞生长的促进作用 |
| Promoting effect of VEGF and BMP-2 on growth of osteoblasts |
| 投稿时间：2014-01-27 |
| DOI：10.3969/j.issn.1674-8573.2014.02.003 |
| 中文关键词: 血管内皮生长因子类 骨形态发生蛋白质类 间质干细胞 成骨细胞 |
| 英文关键词: Vascular endothelial growth factors Bone morphogenetic proteins Mesenchymal stem cells Osteoblasts |
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| 中文摘要: |
| 目的 研究血管内皮生长因子（vascular endothelial growth factor，VEGF）以及骨形态发生蛋白2（bone morphogendtic protein 2，BMP-2）对成骨细胞增殖的调节作用。方法 提取人骨髓间充质干细胞（mesenchymal stem cells，MSCs）并使其分化为成骨细胞，分别添加不同浓度的VEGF和BMP-2进行药物干预，用四甲基偶氮唑盐（MTT）法研究成骨细胞的增殖率。结果 两组干预组的吸光度（A）值与对照组的A值相比较，差异有统计学意义（P<0.05），对成骨细胞增殖的调节作用均随剂量和时间变化而改变，两组调节作用的最佳时间均为72 h左右。两组最佳作用浓度分别为：BMP-2为1.8×10-9mol/L，对成骨细胞的增殖率达179.59%；VEGF为1×10-8mol/L，其增殖率达189.80%。结论 VEGF对成骨细胞增殖的促进作用不亚于BMP-2。 |
| 英文摘要: |
| Objective To study the regulatory effects of vascular endothelial growth factor (VEGF) and bone morphogendtic protein 2 (BMP-2) on proliferation of osteoblasts. Methods The human bone marrow mesenchymal stem cells (MSCs) were isolated and differentiated into osteoblasts, and different concentrations of VEGF and BMP-2 were added for drug intervention. MTT assay was used to examine the proliferation of osteoblasts. Results There was significant difference in aborbance values between intervention group and control group (P<0.05). The regulatory effects of VEGF and BMP-2 on proliferation of osteoblasts were time- and concentration-dependent. The optimal time was about 72 h in both two groups. BMP-2 1.8×10-9mol/L obtained proliferation rate of 179.59%, and VEGF 1×10-8mol/L obtained proliferation rate of 189.80%. Conclusion The promoting effect of VEGF on proliferation of osteoblasts is similar to that of BMP-2. |
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