| 秦安辉,白信信,马腾,等.麦角硫因通过调控衰老相关的神经免疫微环境促进周围神经修复.骨科,2026,17(2): 97-104. |
| 麦角硫因通过调控衰老相关的神经免疫微环境促进周围神经修复 |
| Ergothioneine promotes peripheral nerve repair by modulating the age-related neuroimmune microenvironment |
| 投稿时间：2025-12-19 |
| DOI：10.3969/j.issn.1674-8573.2026.02.001 |
| CN KeyWords: 周围神经再生 衰老 麦角硫因 背根神经节 |
| EN KeyWords: Peripheral nerve regeneration Aging Ergothioneine Dorsal root ganglia |
| Fund Project:国家自然科学基金面上项目（82472417）；陕西省重点研发计划项目（2025SF-YBXM-505） |
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| CN Abstract: |
| 目的 探讨外源性补充麦角硫因（ergothioneine，EGT）对衰老相关周围神经再生障碍的改善作用及其潜在机制。方法 选用18月龄C57小鼠，采用EGT（20 mg/kg）灌胃预处理4周后建立坐骨神经挤压伤模型，并持续干预至损伤后28天。通过免疫荧光、荧光金逆行示踪技术评估EGT对神经早期轴突再生的影响；利用透射电镜、表皮神经支配染色、马松染色及步态分析，评价EGT对长期神经修复及功能性再支配的效果。同时，应用转录组对EGT干预后的背根神经节（dorsal root ganglia，DRG）进行测序，探究EGT调控神经再生的分子及细胞学机制。结果 EGT显著促进了衰老小鼠损伤后早期轴突再生；远期评估显示，EGT有效促进了靶器官的功能性再支配，并减轻了神经源性肌肉萎缩。转录组测序结果表明，EGT显著抑制了损伤后DRG组织中的炎症相关信号通路激活。结论 EGT能够通过抑制衰老个体神经损伤后DRG局部炎症反应，优化再生微环境，从而有效促进周围神经再生，抵抗衰老相关的再生障碍，为衰老相关神经再生障碍的干预提供了新思路。 |
| EN Abstract: |
| Objective To investigate the therapeutic potential and underlying mechanisms of exogenous ergothioneine (EGT) in overcoming age-associated impairments in peripheral nerve regeneration. Methods Eighteen-month-old C57BL/6J mice were pre-treated with EGT (20 mg/kg, gavage) for 4 weeks before undergoing sciatic nerve crush injury, with treatment continuing for another 28 days post-injury. Early axonal regeneration was assessed using immunofluorescence and retrograde tracing. Long-term nerve regeneration and target organ reinnervation of EGT were evaluated via transmission electron microscopy, histological staining and gait analysis. Finally, RNA sequencing (RNA-seq) of dorsal root ganglia (DRG) was performed to elucidate the molecular mechanisms. Results EGT pre-treatment significantly promoted early axonal regrowth across the injury site in aged mice. Long-term assessments revealed that EGT enhanced functional reinnervation of target organs and attenuated neurogenic muscle atrophy. Mechanistically, RNA-seq analysis of the DRG demonstrated that EGT markedly suppressed the activation of inflammation-related signaling pathways following nerve injury. Conclusion EGT effectively counteracts age-related regenerative decline by suppressing local neuroinflammation in the DRG, thereby optimizing the regenerative microenvironment. This study identifies EGT as a promising therapeutic strategy for improving peripheral nerve repair in the elderly. |
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