| 鲁锐,瞿云昆,杨卿,等.酸浆苦味A通过抗炎和抗凋亡途径延缓骨关节炎的进展.骨科,2022,13(5): 426-432. |
| 酸浆苦味A通过抗炎和抗凋亡途径延缓骨关节炎的进展 |
| Physalin A Delays the Progression of Osteoarthritis through Anti-inflammatory and Anti-apoptotic Pathways |
| 投稿时间：2022-05-04 |
| DOI：10.3969/j.issn.1674-8573.2022.05.009 |
| 中文关键词: 酸浆苦味A 骨关节炎 软骨细胞 抗炎 凋亡 |
| 英文关键词: Physalin A Osteoarthritis Chondrocyte Anti-inflammatory Apoptosis |
| 基金项目:国家自然科学基金（81601951） |
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| 中文摘要: |
| 目的 探究酸浆苦味A（Physalin A，PA）对骨关节炎的保护作用及作用机制。方法 取5日龄的C57BL/6乳鼠膝关节原代软骨细胞进行传代培养，使用5 ng/mL的白细胞介素-1β（IL-1β）刺激原代软骨细胞以模拟骨关节炎的软骨细胞炎性模型，并使用不同浓度的PA（2.5 μmol/L、5 μmol/L、10 μmol/L）进行干预，具体分组为对照组、IL-1β组、IL-1β+PA（2.5 μmol/L）组、IL-1β+PA（5 μmol/L）组、IL-1β+PA（10 μmol/L）组。Western blot法检测不同组间蛋白聚糖（ACAN）、二型胶原（COL2A1）、基质金属蛋白酶13（MMP13）、Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶（ADAMTS5）、诱导型一氧化氮合酶（iNOS）、环氧合酶2（COX-2）、白细胞介素6（IL-6）和B淋巴细胞瘤-2（Bcl-2）、Bcl-2相关X蛋白（Bax）的表达水平。结果 与对照组比较，IL-1β组ACAN、COL2A1蛋白表达水平明显下调（P＜0.05），而MMP13、ADAMTS5和iNOS、COX-2、IL-6蛋白表达水平显著升高（P＜0.05）；相比于IL-1β组，IL-1β+PA（2.5 μmol/L）组、IL-1β+PA（5 μmol/L）组、IL-1β+PA（10 μmol/L）组中ACAN、COL2A1蛋白表达水平逐渐升高（P＜0.05），MMP13、ADAMTS5蛋白和iNOS、COX-2、IL-6蛋白表达水平逐渐下降（P＜0.05）。此外，相比于对照组，IL-1β组Bcl-2/Bax比值下降（P＜0.05）；相比于IL-1β组，IL-1β+PA（2.5 μmol/L）组、IL-1β+PA（5 μmol/L）组、IL-1β+PA（10 μmol/L）组中，Bcl-2/Bax比值升高（P＜0.05）。结论 PA能通过抗炎和抗凋亡途径发挥保护关节软骨的作用。 |
| 英文摘要: |
| Objective To investigate the protective effect and mechanism of Physalin A on osteoarthritis. Methods The effects of PA on chondrocytes were studied in vitro. Primary chondrocytes were isolated from the knee joints of 5-day-old C57BL/6 neonatal mice. The cells were stimulated with 5 ng/mL interleukin-1β (IL-1β) to mimic the chondrocyte inflammatory model of osteoarthritis, along with the administration of different concentrations of PA (2.5, 5 and 10 μmol/L), serving as the control group, the IL-1β group, the IL-1β+PA (2.5 μmol/L) group, the IL-1β+PA (5 μmol/L) group, and the IL-1β+PA (10 μmol/L) group, respectively. Western blotting was used to detect the expression levels of ACAN, COL2A1, MMP13, ADAMTS5, iNOS, COX-2, IL-6, Bcl-2 and Bax of chondrocytes among different intervention groups. Results Compared with the control group, the expression levels of ACAN and COL2A1 in chondrocytes in the IL-1β group were significantly down-regulated (P＜0.05), and the expression levels of MMP13, ADAMTS5, iNOS, COX-2 and IL-6 were significantly up-regulated (P＜0.05). As compared with the IL-1β group, the ACAN and COL2A1 were increased, and the MMP13, ADAMTS5, iNOS, COX-2, IL-6 were decreased in the IL-1β+PA (2.5 μmol/L) group, IL-1β+PA (5 μmol/L) group, and the IL-1β+PA (10 μmol/L) group (P＜0.05). In addition, compared with the control group, the Bcl-2/Bax ratio in chondrocytes in the IL-1β group decreased (P＜0.05). As compared with IL-1β group, the Bcl-2/Bax ratio was increased in the IL-1β+PA (2.5 μmol/L) group, IL-1β+PA (5 μmol/L) group, and the IL-1β+PA (10 μmol/L) group (P＜0.05). Conclusion PA protects articular cartilage through anti-inflammatory and anti-apoptotic pathways. |
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