| 宋志文,马阳光,左晓霜,等.体内埋置光纤介导的光生物调节对脊髓损伤后小鼠继发性炎症反应和功能恢复的影响.骨科,2022,13(5): 418-425. |
| 体内埋置光纤介导的光生物调节对脊髓损伤后小鼠继发性炎症反应和功能恢复的影响 |
| Effects of Embedded Optical Fiber-Mediated Photobiomodulation on Secondary Inflammatory Response and Functional Recovery in Mice after Spinal Cord Injury |
| 投稿时间：2022-05-24 |
| DOI：10.3969/j.issn.1674-8573.2022.05.008 |
| 中文关键词: 脊髓损伤 体内埋置光纤 光生物调节 炎症因子 神经修复 |
| 英文关键词: Spinal cord injury Embedded optical fiber Photobiomodulation Inflammatory factors Neural repair |
| 基金项目:陕西省重点研发计划项目（2021ZDLSF02-10，2020ZDLSF02-05） |
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| 中文摘要: |
| 目的 研究体内埋置光纤介导的光生物调节对脊髓损伤小鼠组织修复和运动功能恢复的影响。方法 构建小鼠T9节段钳夹损伤及光生物调节治疗模型，将小鼠随机分为假手术（Sham）组、脊髓损伤（SCI）组、光生物调节治疗（SCI+PBM）组。用实时荧光定量PCR（qPCR）和酶联免疫吸附测定（ELISA）法检测损伤区炎症因子的表达情况。免疫荧光染色及原位末端标记（TUNEL）法观察损伤区神经元存活和轴突再生情况。采用巴索小鼠量表（Basso mouse scale，BMS）及足印记评估脊髓损伤小鼠后肢运动功能恢复情况。结果 与SCI组比较，SCI+PBM组小鼠损伤区的白细胞介素-1α（interleukin-1α，IL-1α）、白细胞介素-1β（interleukin-1β，IL-1β）和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的表达水平显著降低（P＜0.01），总凋亡和神经元凋亡百分比显著降低（P＜0.001），轴突到损伤中心的距离显著缩小（P＜0.001），BMS评分和足印记步长明显优于SCI组（P＜0.01）。结论 体内埋置光纤介导的光生物调节可抑制小鼠脊髓损伤区继发性炎症反应，促进损伤区神经元存活及轴突再生，并最终促使损伤小鼠后肢运动功能的恢复。 |
| 英文摘要: |
| Objective To study the effect of embedded optical fiber-mediated photobiomodulation (PBM) on tissue repair and motor function recovery in mice with spinal cord injury (SCI). Methods The mouse model of T9 segment clamp injury and photobiomodulation therapy was constructed, and the mice were randomly divided into sham operation (Sham) group, SCI group, and SCI+PBM group. The expression of inflammatory factors in the injured area was detected by qPCR and ELISA. Immunofluorescence staining and in situ end labeling (TUNEL) were used to observe neuronal survival and axon regeneration in the injured area. Basso mouse scale (BMS) and footprints were used to evaluate the recovery of motor function of hindlimb in mice with SCI. Results Compared with SCI group, interleukin-1α (IL-1α), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) expression level in the injured area of mice in SCI+PBM group decreased (P＜0.01), and the total apoptosis and the percentage of neuronal apoptosis were significantly decreased (P＜0.001), the distance from the axon to the injury center was significantly reduced (P＜0.001), and the BMS score and footprint step length were significantly improved as compared (P＜0.01). Conclusion In vivo embedded optical fiber-mediated PBM can inhibit the secondary inflammatory response in mice with SCI, promote neuronal survival and axonal regeneration in the injured area, and ultimately promote the recovery of hindlimb motor function in injured mice. |
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