| 张放,孙怡芸,梁晓倩,等.白细胞介素-23通过Smad4调控间充质干细胞成骨分化的机制研究.骨科,2019,10(6): 544-550. |
| 白细胞介素-23通过Smad4调控间充质干细胞成骨分化的机制研究 |
| Osteogenic differentiation of mesenchymal stem cells modulated by interleukin-23 through Smad4 |
| 投稿时间：2019-03-11 |
| DOI：10.3969/j.issn.1674-8573.2019.06.012 |
| 中文关键词: 间充质干细胞 白细胞介素23 成骨分化 |
| 英文关键词: Mesenchymal stem cells Interleukin-23 Osteogenic differentiation |
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| 中文摘要: |
| 目的 研究白细胞介素23（interleukin-23，IL-23）对间充质干细胞（mesenchymal stem cells，MSC）成骨分化的作用，并探讨其机制。方法 分离培养小鼠骨髓MSC，在IL-23刺激下进行成骨诱导分化，通过茜素红实验与碱性磷酸酶实验明确其成骨分化能力；通过全基因组表达谱芯片检测IL-23刺激下MSC成骨分化过程中的基因表达谱，筛选IL-23调控MSC成骨分化的关键基因，并通过荧光定量PCR加以验证；通过siRNA干扰关键基因Smad4表达，并用茜素红实验与碱性磷酸酶实验明确MSC成骨分化能力改变。结果 在成骨诱导分化条件下，IL-23刺激的MSC茜素红实验与碱性磷酸酶实验结果均显著高于无IL-23刺激的对照组；全基因组表达谱芯片结果显示，IL-23刺激下MSC成骨分化过程中Smad4表达明显上调；siRNA干扰Smad4表达后，IL-23刺激引起的MSC成骨能力增强被明显抑制。结论 IL-23通过上调Smad4表达以促进MSC成骨分化。 |
| 英文摘要: |
| Objective To study the effects of interleukin-23 (IL-23) on the osteogenic differentiation of mesenchymal stem cells (MSCs) and the possible mechanism. Methods Bone marrow-derived MSCs were separated and cultured. MSCs were treated with IL-23, and induced to osteogenic differentiation. The osteogenic differentiation ability was determined by the alizarin red and alkaline phosphatase assays. In order to screen the key gene, the expression profile of MSCs treated with IL-23 was detected by whole genome expression spectrum chip and then confirmed by qRT-PCR. Smad4 was identified and inhibited by siRNA, and the alizarin red and alkaline phosphatase assays were performed to determine the change of osteogenic differentiation ability of MSCs. Results With the induction of osteogenic differentiation, the osteogenic differentiation ability of MSCs in IL-23-treated group was significantly greater than that in control group. The Smad4 expression in IL-23-treated group was significantly up-regulated as compared with that in control group. Inhibiting Smad4 by siRNA could rectify the enhanced osteogenic differentiation ability of MSCs caused by IL-23. Conclusion IL-23 promotes the osteogenic differentiation of MSCs through up-regulating the Smad4 expression. |
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