| 马溢聪,徐文飞,刘凤,等.鲨鱼软骨粉抑制白细胞介素-1β诱导的人软骨细胞凋亡的实验研究.骨科,2019,10(3): 210-216. |
| 鲨鱼软骨粉抑制白细胞介素-1β诱导的人软骨细胞凋亡的实验研究 |
| Experimental study on inhibitory effects of shark cartilage on interleukin-1β-induced apoptosis of human chondrocytes |
| 投稿时间：2019-02-23 |
| DOI：10.3969/j.issn.1674-8573.2019.03.009 |
| 中文关键词: 鲨鱼软骨粉 白细胞介素-1β 软骨细胞 凋亡 |
| 英文关键词: Shark cartilage Interleukin-1β Chondrocytes Apoptosis |
| 基金项目:国家自然科学基金（31770849、31500640）；浙江省自然科学基金（LY15C070002、LY16C050001）；嘉兴市科技计划项目（2015AY23007） |
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| 中文摘要: |
| 目的 探究鲨鱼软骨粉对白细胞介素（IL）-1β诱导的人软骨细胞凋亡的影响及其可能的机制。方法 分别用浓度为0.1、0.2、1、5、10 mg/ml的鲨鱼软骨粉溶液干预软骨细胞48 h，采用MTS法检测不同浓度的鲨鱼软骨粉溶液对软骨细胞增殖的影响。将软骨细胞分为3组，对照组只加入培养基；IL-1β组，加入10 ng/ml IL-1β；鲨鱼软骨粉组，加入10 ng/ml IL-1β和5 mg/ml鲨鱼软骨粉溶液。流式细胞术检测各组细胞凋亡及细胞周期分布情况，Western blot检测signal transducers and activators of transcription 3（STAT3），p65， extracellular regulated protein kinases（ERK），p-STAT3，p-p65，p-ERK，B-cell lymphoma-extra large（Bcl-xl）和Survivin表达情况。结果 鲨鱼软骨粉溶液能明显促进软骨细胞生长，且浓度为5 mg/ml时效果最明显（P＜0.001）。与IL-1β组比较，鲨鱼软骨粉组细胞凋亡明显减少（P＜0.001），STAT3， p65，ERK磷酸化明显减少（P＜0.05，P＜0.001，P＜0.05），Bcl-xl、Survivin蛋白表达增加（P均＜0.01）。结论 鲨鱼软骨粉溶液能够抑制STAT3，p65，ERK磷酸化，并增加Bcl-xl、Survivin蛋白表达，从而抑制软骨细胞凋亡。 |
| 英文摘要: |
| Objective To investigate the effects of shark cartilage on interleukin-1β (IL-1β)-induced apoptosis of human chondrocytes and possible mechanisms. Methods The effect of different concentrations of shark cartilage solution (0.1, 2, 1, 5 and 10 mg/ml) on the proliferation of human chondrocytes was measured by MTS method. The chondrocytes were divided into 3 groups:the control group was given the medium only; the IL-1β group was treated with 10 ng/ml IL-1β; the shark cartilage group was treated with 10 ng/ml IL-1β and 5 mg/ml shark cartilage solution. The apoptosis and cell cycle of each group were examined by flow cytometry, and the expression of signal transducers and activators of transcription 3 (STAT3), p65, extracellular regulated protein kinases (ERK), p-STAT3, p-p65, p-ERK, B-cell lymphoma-extra large (Bcl-xl) and Survivin was detected by Western blot. Results Shark cartilage solution could significantly promote the chondrocytes growth and 5 mg/ml presented the most obvious effect (P＜0.001). As compared with IL-1β group, the cell apoptosis in shark cartilage group was significantly reduced (P＜0.001), phosphorylation of STAT3, p65 and ERK significantly decreased (P＜0.05, P＜0.001, and P＜0.05 respectively), and the expression of Bcl-xl and Survivin protein increased (both P＜0.01). Conclusion Shark cartilage solution can inhibit apoptosis of chondrocytes by inhibiting STAT3, p65 and ERK phosphorylation, and increasing the expression of Bcl-xl and Survivin proteins. |
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