文章摘要
李晓娟,李浩,马永壮,闫吉元,张俊,马天,刘朝旭,杨勇,任晔,吴华.缺氧环境通过HIF-1α/YAP信号促进大鼠生长板软骨细胞表型维持.骨科,2019,10(2):134-139
缺氧环境通过HIF-1α/YAP信号促进大鼠生长板软骨细胞表型维持
Hypoxia promotes the maintenance of chondrogenic phenotype in rat growth plate chondrocytes through the HIF-1α/YAP signaling
投稿时间:2019-01-21  
DOI:10.3969/j.issn.1674-8573.2019.02.011
中文关键词: 氯化钴  缺氧诱导因子-1α  YAP  软骨表型
英文关键词: Cobalt chloride  HIF-1α  YAP  Chondrogenic phenotype
基金项目:国家自然科学基金(51537004)
作者单位E-mail
李晓娟 华中科技大学同济医学院附属同济医院肾内科武汉 430030  
李浩 华中科技大学同济医学院附属同济医院骨科武汉 430030  
马永壮 华中科技大学同济医学院附属同济医院骨科武汉 430030  
闫吉元 华中科技大学同济医学院附属同济医院骨科武汉 430030  
张俊 华中科技大学同济医学院附属同济医院骨科武汉 430030  
马天 华中科技大学同济医学院附属同济医院骨科武汉 430030  
刘朝旭 华中科技大学同济医学院附属同济医院骨科武汉 430030  
杨勇 华中科技大学同济医学院附属同济医院骨科武汉 430030  
任晔 华中科技大学同济医学院附属同济医院骨科武汉 430030  
吴华 华中科技大学同济医学院附属同济医院骨科武汉 430030 wuhua360@aliyun.com 
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中文摘要:
      目的 研究缺氧条件下缺氧诱导因子-1α(hypoxia inducible factor-1α, HIF-1α)和Yes相关蛋白(Yes-associated protein, YAP)蛋白在软骨细胞表型维持中的作用。方法 生长板软骨细胞取自7日龄的SD大鼠,并通过氯化钴干预以模拟体外缺氧微环境。通过检测细胞外基质水平和Ⅱ型胶原(Collagen 2, Col2)蛋白水平表达变化来确定软骨细胞在氯化钴刺激下软骨表型变化。通过Western blotting方法检测HIF-1α、YAP蛋白水平在氯化钴刺激后的表达变化。结果 氯化钴于体外实验中模拟的缺氧环境能够促进软骨细胞细胞外基质的表达水平,并且能够上调Col2蛋白表达,同时上调HIF-1α和YAP蛋白水平的表达。抑制HIF-1α的表达可以下调缺氧环境所致的YAP蛋白表达水平的上调,而抑制YAP的表达并不能影响HIF-1α蛋白表达水平。结论 氯化钴于体外实验中模拟的缺氧环境可能通过HIF-1α/YAP信号促进大鼠生长板软骨细胞软骨表型的维持。
英文摘要:
      Objective To investigate the role of hypoxia inducible factor-1α (HIF-1α) and Yes-associated protein (YAP) in chondrogenic phenotype maintenance of hypoxia-induced growth plate chondrocytes in vitro. Methods The growth plate chondrocytes were harvested from 7-day-old SD rats and cultured with cobalt chloride for hypoxia simulation. The phenotype changes of chondrocytes stimulated by cobalt chloride were determined by measuring the changes of extracellular matrix levels and the expression levels of Col2 protein. The expression levels of HIF-1α and YAP were detected by Western blotting. Results The hypoxia simulated by cobalt chloride could promote the expression level of extracellular matrix of chondrocytes and up-regulate Col2 protein expression. At the same time, cobalt chloride could up-regulate the expression levels of HIF-1α and YAP proteins. Inhibition of HIF-1α expression could down-regulate the up-regulation of YAP protein expression by cobalt chloride, while inhibition of YAP expression did not affect HIF-1α protein expression. Conclusion Under the hypoxia environment simulated by cobalt chloride, the HIF-1α/YAP signaling may play an important role in controlling the maintenance of the chondrogenic phenotype in growth plate chondrocytes.
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