| 李景峰,陈舒振,杨阳,等.SOX5对骨肉瘤细胞迁移和侵袭能力的影响.骨科,2017,8(4): 309-312,316. |
| SOX5对骨肉瘤细胞迁移和侵袭能力的影响 |
| Effect of SOX5 on migaration and invasion of osteosarcoma MG63 cells |
| 投稿时间：2016-12-17 |
| DOI：10.3969/j.issn.1674-8573.2017.04.012 |
| 中文关键词: SOX5 骨肉瘤 迁移 侵袭 |
| 英文关键词: SOX5 Osteosarcoma Migration Invasion |
| 基金项目:湖北省科技计划项目（2016CFB570） |
|
| 摘要点击次数: 4588 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 观察Y染色体上的性别决定区相关高迁移率族盒蛋白-5（sex determining region Y-box protein 5, SOX5）对骨肉瘤细胞MG63迁移和侵袭能力的影响，探讨其在骨肉瘤发病和转移中的作用。方法 本研究为前瞻性研究，通过脂质体介导的基因转染技术将SOX5的三个不同的siRNA（siSOX5-1、siSOX5-2和siSOX5-3）瞬时转染骨肉瘤细胞MG63，转染后48 h检测SOX5的mRNA和蛋白表达，从中挑选出干扰效果最明显的siRNA。进而采用博伊登室实验方法分别对干扰前后的骨肉瘤细胞MG63进行体外细胞迁移和侵袭测定，采用免疫印迹（Western blot）检测迁移和侵袭相关蛋白［基质金属蛋白酶（matrix metallproteinase, MMP）-2、MMP-9、Twist1和Snail1）］的表达水平。结果 3条针对人的siRNA（siSOX5-1、siSOX5-2、siSOX5-3）均能够降低SOX5 mRNA及蛋白水平表达，其中siSOX5-3干扰效果最明显。siSOX5-3干扰组的迁移细胞数为（52±5）较对照组的（107±9）显著减少（P＜0.05），siSOX5-3干扰组的侵袭细胞数为（27±4）较对照组的（71±2）显著减少（P＜0.05）。Western blot结果显示siSOX5-3干扰组的侵袭相关蛋白（MMP-2、MMP-9、Twist1和Snail1）的表达均较对照组显著降低（P＜0.05）。结论 SOX5 RNA干扰能显著降低骨肉瘤细胞的迁移和侵袭能力，SOX5能促进骨肉瘤细胞的迁移和侵袭。 |
| 英文摘要: |
| Objective To observe the effect of RNA interference of sex determining region Y-box protein 5 (SOX5) on migration and invasion of MG63 cells, and reveal its role in the pathogenesis and metastasis of osteosarcoma. Methods This study was a prospective study. MG63 cells were transiently transfected with the siRNAs by using Liposome-mediated gene transfection. The mRNA and protein expression of SOX5 was detected 48 h after transfection. Then the most obvious interference efficacy of siRNA was selected. The migration and invasion of MG63 cells were detected by Boyden chambers. The expression level of MMP-2, MMP-9, Twist1 and Snail1 was detected by Western blotting. Results All the three siRNAs targeting SOX5 (siSOX5-1, siSOX5-2, siSOX5-3) could decrease the SOX5 mRNA and protein levels, but siSOX5-3 was the most effective one among the tested siRNAs. SOX5 knockdown significantly reduced the number of migratory MG63 cells (52±5) as compared with control group (107±9) (P＜0.05), and that of invasive MG63 cells (27±4) as compared with control group (71±2) (P＜0.05). Low expression of SOX5 reduced the expression level of MMP-2, MMP-9, Twist1 and Snail1 (P＜0.05). Conclusion SOX5 RNA interference can significantly reduce the migration and invasion of osteosarcoma cells, and SOX5 can promote the migration and invasion of osteosarcoma cells. |
|
查看全文
下载PDF阅读器 |
| 关闭 |
|
|
|
