| 王威,廖苏平,危蕾,等.纳米纤维支架对人肌腱成纤维细胞的生长及相关基因表达的影响.骨科,2015,6(1): 1-5. |
| 纳米纤维支架对人肌腱成纤维细胞的生长及相关基因表达的影响 |
| Influence of PLLA/Col-Ⅰ nanofibers on growth of human tendon-derived fibroblast and the gene expression |
| 投稿时间：2014-08-06 |
| DOI：10.3969/j.issn.1674-8573.2015.01.001 |
| 中文关键词: 肌腱 成纤维细胞 纳米纤维 聚乳酸 Ⅰ型胶原蛋白 |
| 英文关键词: Tendon Fibroblast Nanofibers Poly-L-lactic acid Collagen Ⅰ |
| 基金项目:武汉市卫计委临床医学科研项目（No.WX11C20） |
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| 中文摘要: |
| 目的 研究人肌腱成纤维细胞（tendon derived fibroblasts, TDFs）在聚乳酸（poly-L-lactic acid, PLLA）纳米纤维支架及聚乳酸/Ⅰ型胶原蛋白（PLLA/Col-Ⅰ）纳米纤维支架上的生长情况及相关基因的表达情况。
方法 将TDFs分别种植于空白盖玻片（空白组）、载有PLLA纳米纤维支架的盖玻片（PLLA组）和载有PLLA/Col-Ⅰ纳米纤维支架的盖玻片上（PLLA/Col-Ⅰ组），观察细胞生长情况，并检测Ⅰ、Ⅲ、Ⅹ型胶原蛋白（Col-Ⅰ、Col-Ⅲ、Col-Ⅹ）以及整合素相关基因黏着斑激酶(focal adhesion kinase, FAK)的基因表达情况。
结果 PLLA组细胞生长速度低于空白组和PLLA/Col-Ⅰ组，而PLLA/Col-Ⅰ组和空白组相比，细胞生长速度差异无统计学意义。和PLLA组及空白组相比，PLLA/Col-Ⅰ组中Col-Ⅰ、Col-Ⅲ、Col-Ⅹ以及FAK基因表达水平显著升高，PLLA组和空白组无明显区别。结论 PLLA/Col-Ⅰ纳米纤维支架对TDFs的生长有促进作用，且有利于Col-Ⅰ、Col-Ⅲ、Col-Ⅹ及FAK基因的表达，能为肌腱修复提供一种理想的组织工程支架材料。 |
| 英文摘要: |
| Objective To explore the effects of PLLA nanofibers and PLLA/Col-Ⅰ nanofibers on human tendon-derived fibroblasts (TDFs), and the related gene expression. Methods TDFs were respectively cultured on the cover slip (blank group), PLLA nanofibers (PLLA group) and PLLA/Col-Ⅰ nanofibers (PLLA/Col-Ⅰ group). The growth of TDFs, and the gene expression of collagensⅠ, Ⅲ, Ⅹ and FAK during the course of culture were analyzed. Results The growth of TDFs in PLLA group was significantly inhibited as compared with blank group and PLLA/Col-Ⅰ group. The gene expression of collagen Ⅰ, collagen Ⅲ and collagen Ⅹ, and FAK was remarkably up-regulated in PLLA/Col-Ⅰ group as compared with PLLA group and blank group, but there was no significant difference between PLLA group and blank group. Conclusion This study indicates that PLLA/Col-Ⅰ nanofibers can promote the growth of TDFs, and can enhance the gene expression of collagen Ⅰ, collagen Ⅲ and collagen Ⅹ remarkably, as well as the gene expression of FAK. PLLA/Col-Ⅰ nanofibers may be an ideal scaffold of tissue engineering technology in the repair of tendon. |
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